Translational_Unit

Part:BBa_K1505001:Design

Designed by: Renyao Wei   Group: iGEM14_Penn   (2014-10-10)


RBS+smtA+mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 7
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We researched the optimum linker sequence for fusion protein to facilitate successful fusion. The mCherry protein is expected to improve the folding of SmtA (from E.coli) in AMB-1.


Source

RBS is predicted from pmsp3 promoter from Magnetospirillum magneticum strain AMB-1 genome. smtA sequence is from BioBrick part BBa_K190021 (we modified the illegal PstI site).

References